Research Article

Ontogenetic Survey of Histone Modifications in an Annelid

Figure 2

H3K14 acetylation in early development of Polydora cornuta. (a, b) Companion micrographs of an oocyte inside the coelom of a female in paraffin section showing nuclear DNA (a, DAPI) and nuclei that are acetylated at H3K14 (b, FITC-conjugated anti-H3K14ac). The remaining images are bright field (left) and companion images showing DNA (DAPI, middle) and nuclei with H3K14ac (FITC-conjugated anti-H3K14ac, right). (c–e) Two-cell stage with polar bodies and a polar lobe (P-brood). (f–h) Eight-cell embryo, shown from the animal pole (P-brood). ch: chaetae, D: D macromere, fc: follicular cell, ma: macromere, mi: micromere, n: nucleus, on: oocyte nucleus, pb: polar body, and pl: polar lobe. Scale bars = 50 μm.
392903.fig.002a
(a)
392903.fig.002b
(b)
392903.fig.002c
(c)
392903.fig.002d
(d)
392903.fig.002e
(e)
392903.fig.002f
(f)
392903.fig.002g
(g)
392903.fig.002h
(h)