Genetics Research International / 2012 / Article / Tab 2

Review Article

Mechanistic Roles of Noncoding RNAs in Lung Cancer Biology and Their Clinical Implications

Table 2

Involvement of miRNAs in lung cancer and technologies used for identification.

miRNA affectedSignificanceTechnologySource tissueReferences

Overexpression of miR-155, miR-21, and miR-106a.
Decreased expression of let-7a
Prognostic biomarker of adenocarcinoma patient survivalOligonucleotide microchipPrimary LC cases and corresponding noncancerous tissues[12]
Overexpression of miR-21Candidate for molecular targets in treatment for LC in never-smokers miRNA microarray assay on a CodeLink platform (miRNA oligo probe)Matched pairs of LC and noncancerous lung tissues from never-smokers[134]
Decreased expression of let-7Shortened postoperative survival in NSCLCRT-PCRTumor specimens[30]
Decreased expression of miRNA-451Expression negatively associated with lymph node metastasis, the stage of TNM classification and poor prognosis of NSCLC patientsqPCR, confirmed by northern blot analysisFresh tissue of NSCLC samples and the adjacent histologically normal tissue.[171]
Overexpression of miR-92-1Regulation of RAB14 gene at the translational level.
This might cause a decrease in the lung surfactant secretion, and loss of the protection of lung cells against external carcinogens
2D electrophoresis profiling and mass spectrometric analysisSBC-3 cell line[172]
Decreased expression of miR-30aMay function as a tumor suppressor, by targeting Snai1 and inhibiting migration, invasion, and metastasisqPCRA549 cell line and fresh snap-frozen surgical specimens of tumor tissues and of the corresponding normal specimens[173]
Overexpression of miR-21Overexpressed in tumor tissues relative to adjacent nontumor tissues.
Negative regulation of PTEN and promotion of cellular growth and invasion in NSCLC cells
qRT-PCRPaired NSCLC and adjacent non-tumor lung tissues[174]
Overexpression of miR-126Inhibition of tumor growth in vivo by targeting EGFL7Flow cytometry assay, qRT-PCR, and Western blot A549 cell line[175]
Decreased expression of miR-133BIncreased apoptosis in response to gemcitabine and reduced MCL-1 and BCL2L2 expressionQuantitative-reverse transcriptase (q-RT) PCR profilingFrozen lung tumors (adenocarcinoma) and noninvolved adjacent lung and LC cell lines[78]
Overexpression and gain of miR-17-92Enhanced cell proliferationNorthern blot confirmed with RT-PCR, followed by southern blotSCLC tissue and cell lines[55]
Loss of miR-1Inhibition of cell proliferation and invasion in vitro, and tumor growth in vivo, by targeting MET and FoxP1qRT-PCRLung cancer tissue and cell lines[59]
Hypermethylation of miR-34aAvoidance of senescenceMethylation-specific PCR followed by qRT-PCRLung cancer cell lines[57]
Overexpression of miR-25 and miR-223Biomarker of NSCLC found in sera of NSCLC patients, but not in that of healthy donorsSolexa sequencingSera from NSCLC compared to healthy donor controls[127]
Overexpression of miR-21 and miR-210, decreased expression of miR-486-5pBiomarkers of malignant nodules identified by CTqRT-PCRPlasma from patients with malignant nodules compared to those with benign nodules and healthy controls[133]

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