Association Study between Idiopathic Scoliosis and Polymorphic Variants of VDR, IGF-1, and AMPD1 Genes

Table 2

PCR-RFLP protocol.

Gene, polymorphism	Annealing temperature, °C	PCR product size, bp	Restriction enzyme	Restriction fragments, bp
VDR (rs2228670)	59	265	FokI	FF: 265; Ff: 265 + 196 + 69; ff: 196 + 69
IGF-1 (rs5742612)	59	330	BslI	TT: 330; CT: 330 + 277 + 53; CC: 277 + 53
AMPD1 (rs17602729)	58	136	MaeII	CC: 97 + 39; CT: 136 + 97 + 39; TT: 136

PCR indicates polymerase chain reaction; RFLP: restriction fragment length polymorphism; bp: base pair; VDR: vitamin D receptor; IGF-1: insulin-like growth factor-1; AMPD1: adenosine monophosphate deaminase 1.