The Noncell Autonomous Requirement of Proboscipedia for Growth and Differentiation of the Distal Maxillary Palp during Metamorphosis of Drosophila melanogaster

Figure 3

The maxillary palpus phenotypes of three independent mosaic analyses. Panels (A) and (B) are scanning electron micrographs of the effects of clonal loss of PB function generated in flies with the genotype y w; PhspFLP}/+; Pry⁺, neo^r, FRT}82B pb²⁷/Pry⁺, neo^r, FRT}82B Sb^63b M(3)95A² Py⁺, ry⁺}96E. In panel (A), a Sb⁺ pb²⁷ clone in the distal maxillary palp is shown and the maxillary palp is reduced. In panel (B), Sb pb⁺ distal maxillary palps are shown; the right is wild type, and the left is reduced indicated by the arrow. In panels (A) and (B), the arrows indicate Sb bristles and the arrowheads Sb⁺ bristles. Panels (C) and (D) are bright field micrographs of clonal loss of PB function generated in the genotype y w; PhspFLP}/+; Pry⁺, neo^r, FRT}82B pb²⁷ Scr² Pw⁺, ry⁺}90E/Pry⁺, neo^r, FRT}82B M(3)95A² Py⁺, ry⁺}96E Pexd⁺, w⁺}. Panel (C) is one of the 22 pb²⁷/+ wild type distal maxillary palps with the y⁺ proximal palpus bristle indicated by the arrow and y⁺ maxillary socket tricombs indicated with the arrowhead. Panel (D) shows a y^- pb²⁷Scr² maxillary palpus. Panels (E)–(G) are bright field micrographs of distal maxillary palps from the clonal ectopic expression of PB in a pb²⁷/pb²⁰ mutant background generated in the genotype y w, Pw⁺, pb^a>y⁺>Tubα1}B; PhspFLP}/+; pb²⁷/pb²⁰. Panel (E) is a rescued y^- and PB expressing (pb^a>Tubα1) maxillary palpus, panel (F) is a reduced y^- and PB expressing (pb^a>Tubα1) maxillary palpus, and panel (G) is a reduced y⁺ pb^- (pb^a>y⁺>Tubα1) maxillary palpus.