The TLR4/TRIF-Mediated Activation of NLRP3 Inflammasome Underlies Endotoxin-Induced Liver Injury in Mice
Figure 4
Hepatosplenomegaly and hepatic granulomas in P. acnes-primed mice. Wild-type mice received Cy3-labeled, heat-killed P. acnes, and at day 7 various tissues were removed and weighed (a, b). The liver slices were stained with anti-F4/80 mAb and DAPI for detecting macrophages and cell nuclei, respectively (c). Kupffer cells and splenocytes were prepared from P. acnes-primed (closed) or naïve mice (open). After staining with anti-F4/80 mAb, proportion of cells were determined by FACS, and total cell number was counted.
