Figure 1: Allele-specific PCR analysis of the N34S mutation on exon 3 of the SPINK gene. The wild-type SPINK band is located at 285 bp. If the N34S mutation is present, a second band at 190 bp appears. The lanes 41 to 55 represent patients without the N34S mutation (normal). The lane marked with M represents the 100 bp DNA Ladder (Fermentas Life Sciences, Lithuania).