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Gastroenterology Research and Practice
Volume 2012, Article ID 560345, 6 pages
Research Article

Establishment of a Standardized Liver Fibrosis Model with Different Pathological Stages in Rats

1Ganmei Hospital, Kunming Medical University, Kunming 650000, China
2The Second Affiliated Hospital, Kunming Medical University, Kunming 650000, China
3Molecular Epidemiology Joint Laboratory, Institute of Medical Biology, Kunming 650000, China

Received 1 February 2012; Revised 5 April 2012; Accepted 19 April 2012

Academic Editor: Edoardo G. Giannini

Copyright © 2012 Li Li et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Objective. To establish a standardized animal model for liver fibrosis with the same assessment criteria for liver fibrosis studies that have been established on a unified platform. Methods. The standardized liver fibrosis model was established using Sprague-Dawley (SD) rats that either received an intraperitoneal injection of carbon tetrachloride (CCl4) in small dosages or ingested an ethanol solution. Results. The definite corresponding rules among modeling of different weeks and corresponding serology indices as well as different pathological staging can be observed by modeling with small dosages and slow, individualized, and combined administrations. Conclusion. This method can be used for the standardized establishment of a liver fibrosis model in rats across 5 pathological stages, ranging from S0 to S4, with a high success rate (89.33%) and low death rate (17.3%) because of the application of multiple hypotoxic chemicals for modeling. We refer to the criteria of Histological Grading and Staging of Chronic Hepatitis for Fibrosis established by the 10th World Digestive Disease Academic Conference in Los Angeles in September 1994 (revised in November 2000).