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Gastroenterology Research and Practice
Volume 2012, Article ID 639291, 7 pages
Research Article

Genetically Modified Lactococcus lactis for Delivery of Human Interleukin-10 to Dendritic Cells

1Department of Gastroenterology and Hepatology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
2Department of Medical Microbiology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
3Department of Cell Biology, Histology, and Dermatology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands
4Department of Gastroenterology and Hepatology, Erasmus Medical Center, P.O. Box 2040, 3000 CA, Rotterdam, The Netherlands
5Department of Gastroenterology and Hepatology, Leiden University Medical Center, 2333 ZA Leiden, The Netherlands

Received 4 February 2011; Revised 23 May 2011; Accepted 25 May 2011

Academic Editor: Harold Marcotte

Copyright © 2012 Inge L. Huibregtse et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Interleukin-10 (IL-10) plays an indispensable role in mucosal tolerance by programming dendritic cells (DCs) to induce suppressor Th-cells. We have tested the modulating effect of L. lactis secreting human IL-10 ( ) on DC function in vitro. Monocyte-derived DC incubated with induced effector Th-cells that markedly suppressed the proliferation of allogenic Th-cells as compared to L. lactis. This suppressive effect was only seen when DC showed increased CD83 and CD86 expression. Furthermore, enhanced production of IL-10 was measured in both -derived DC and Th-cells compared to L. lactis-derived DC and Th-cells. Neutralizing IL-10 during DC-Th-cell interaction and coculturing -derived suppressor Th-cells with allogenic Th-cells in a transwell system prevented the induction of suppressor Th-cells. Only 130 pg/mL of bacterial-derived IL-10 and 40 times more exogenously added recombinant human IL-10 were needed during DC priming for the generation of suppressor Th-cells. The spatially restricted delivery of IL-10 by food-grade bacteria is a promising strategy to induce suppressor Th-cells in vivo and to treat inflammatory diseases.