Figure 1: Sulindac sulfide-induced apoptosis is enhanced in serum-free media versus serum-containing media. Equal numbers of HT-29 cells were plated in each well of 6-well plates, allowed to attach overnight, and then incubated in serum-free media and media containing 5% FBS overnight before treatments with vehicle and sulindac sulfide for the indicated durations. Fold apoptosis is determined by measuring caspase-3 activity in total cell lysates. *Indicates significant increase in fold apoptosis relative to 0 hour timepoint, .