Figure 3: Expression of zinc fingers and homeoboxes 2 (ZHX2), nuclear factor-YA (NF-YA), and alpha-fetal protein (AFP) in HepG2 cells after siRNA transfection. The target protein expression was detected by Western blot analysis. The expression of β-actin was analyzed as an internal control. The amounts of protein in the ZHX2 siRNA-treated cells were compared to those of control cells. After siRNA transfection, ZHX2 protein expression (92KD) was decreased by 83%, NF-YA protein expression (40 KD and 43 KD) was reduced by 61%, and AFP protein expression (70 KD) was increased by 2.4-fold.