Research Article

LEPREL1 Expression in Human Hepatocellular Carcinoma and Its Suppressor Role on Cell Proliferation

Figure 1

LEPREL1 was frequently downregulated in HCC. (a) The expression level of LEPREL1 relative to GAPDH was compared between the nontumorous and tumor tissues in 80 HCCs using quantitative RT-PCR. Expression of LEPREL1 in tumor tissues was significantly lower than that of the nontumorous tissues ( , ). (b) Representative pictures of LEPREL1 protein expression in randomly selected paired HCC nontumor and tumor tissues. GAPDH was used as an endogenous control. LEPREL1 was downregulated in most of the tumor tissues as compared to the adjacent nontumorous tissues. ((c), (d)) Expression of LEPREL1 in the tumor tissues and adjacent nontumor tissues. Representative pictures of the immunohistochemistry results of LEPREL1 in tissues (d) and histogram of semiquantitatively with a three-tiered system grades ( ). N: nontumor, T: tumor.