Lung and Intestine: A Specific Link in an Ulcerative Colitis Rat Model
The role of microvasculature in the pathogenesis of ulcerative colitis. (A) Endothelial dysfunction: immunochemistry and RT-PCR results of iNOS in the colon and lung tissue homogenate. (B) Platelet activation: radioimmunoassay results of TXB2, 6-Keto-PGF1α and P-selectin concentration (pg/mL) in the colon and lung tissue homogenate. (C) Leukocyte-endothelium interaction: Immunochemistry and western blotting results of ICAM-1 in the colon and lung tissue homogenate. (D) Angiogenesis: immunochemistry and western blotting results of VEGF-A in the colon and lung tissue homogenate. Band intensities were quantified using Image J software. (a) Normal colon, (b) colon, (c) colon, (d) normal lung, (e) lung, and (f) lung. Compared with the model group: *, ; **, , ***; . Values are means, with error bars indicating SD.