Figure 6: Orthotopic mouse model of BxPC-3 human pancreatic cancer dual labeled with anti-CEA Alexa 488 and 555. (a) Laparoscopic images of representative mouse specimen with BxPC-3 dual labeled with anti-CEA Alexa 488 and 555. The combination of red and green fluorophores creates a significantly brighter fluorescence signal without compromising background illumination. (i–iii) are laparoscopic images of the left upper quadrant. (iv) and (v) are laparoscopic images of metastatic tumor deposits hidden within the mesentery of the mouse. These deposits were virtually undetectable under BL. (b) (i–iii) are intravital OV-100 images of the same mouse specimen under (i) GFPa filter (excitation 460–490; emission 510–550), (ii) RFP filter (excitation BP 535–555; emission 570–623), and (iii) GFP (excitation 460–490; emission 510F) filters. The bottom image (iii) corresponds to GFP bandwidth through which fluorescence laparoscopy is viewed. (iv–vi) are the corresponding intravital Maestro images. (iv) and (v) are spectral unmixing images of the (vi) compositive image obtained through (iv) GFP and (v) RFP filter sets, respectively. These images confirm the dual labeling of BxPC-3 tumor with Alexa 488 and 555 [3].