Figure 2: Flow cytometric analysis to identify aberrant and normal IELs. Cells with a strong CD45 expression and low to intermediate forward and sideward scatter were selected (a), after which IELs expressing intracellular CD3 expression were used for further studies (b). Ab IEL: aberrant IEL population (sCD3-cytCD3+): NL IEL: normal IEL population (sCD3 + cytCD3+).