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Gastroenterology Research and Practice
Volume 2013, Article ID 636785, 13 pages
Research Article

Dominant Fecal Microbiota in Newly Diagnosed Untreated Inflammatory Bowel Disease Patients

1Hedmark University College, 2306 Hamar, Norway
2Department of Chemistry, Biotechnology and Food Science, Norwegian University for Life Sciences, 1432 Ås, Norway
3Department of Gastroenterology, Akershus University Hospital, 1478 Lørenskog, Norway
4Department of Clinical Molecular Biology and Laboratory Sciences (EpiGen), Division of Medicine, Akershus University Hospital, University of Oslo, 0316 Oslo, Norway
5Pediatric Department, Oslo University Hospital, Ullevål, 0450 Oslo, Norway
6Medical Clinic, Oslo University Hospital, Rikshospitalet, 0372 Oslo, Norway

Received 30 April 2013; Revised 15 August 2013; Accepted 29 August 2013

Academic Editor: Akira Andoh

Copyright © 2013 Lill Therese Thorkildsen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Our knowledge about the microbiota associated with the onset of IBD is limited. The aim of our study was to investigate the correlation between IBD and the fecal microbiota for early diagnosed untreated patients. The fecal samples used were a part of the Inflammatory Bowel South-Eastern Norway II (IBSEN II) study and were collected from CD patients ( ), UC patients ( ), unclassified IBD (IBDU) patients ( ), and from a control group ( ). The bacteria associated with the fecal samples were analyzed using a direct 16S rRNA gene-sequencing approach combined with a multivariate curve resolution (MCR) analysis. In addition, a 16S rRNA gene clone library was prepared for the construction of bacteria-specific gene-targeted single nucleotide primer extension (SNuPE) probes. The MCR analysis resulted in the recovery of five pure components of the dominant bacteria present: Escherichia/Shigella, Faecalibacterium, Bacteroides, and two components of unclassified Clostridiales. Escherichia/Shigella was found to be significantly increased in CD patients compared to control subjects, and Faecalibacterium was found to be significantly reduced in CD patients compared to both UC patients and control subjects. Furthermore, a SNuPE probe specific for Escherichia/Shigella showed a significant overrepresentation of Escherichia/Shigella in CD patients compared to control subjects. In conclusion, samples from CD patients exhibited an increase in Escherichia/Shigella and a decrease in Faecalibacterium indicating that the onset of the disease is associated with an increase in proinflammatory and a decrease in anti-inflammatory bacteria.