Difference in Early Activation of NF-κB and MCP-1 in Acinar-Cell-Rich versus Fibrotic Human Pancreas Exposed to Surgical Trauma and Hypoxia
Figure 4
MCP-1 expression in acinar-cell-rich ((a), (b)) and fibrotic ((c), (d)) pancreata. Immediately after sampling, parallel portions of each tissue specimen were immersed in saline and kept at room temperature for 15 minutes ((a), (c)) or 4 hours ((b), (d)), after which they were fixed and processed for immunohistochemical analysis. MCP-1 staining was equally intense in the ductal cells of acinar-cell-rich pancreata after 15 minutes and 4 hours ((a), (b), arrows), whereas intra-acinar MCP-1 expression was observed to slightly increase with time. In fibrotic pancreata, MCP-1 in ductal and acinar cells remained undetectable at 15 minutes (c). At 4 hours, weak staining can be detected in ductal cells ((d), arrow). The slides were counterstained with hematoxylin.
