The Genetic Predisposition and Its Impact on the Diabetes Mellitus Development in Patients with Alcoholic Chronic Pancreatitis

Table 1

Primer sequence and conditions for polymerase chain reaction-sequencing.

Variant	Primer sequence (forward and reverse)	Annealing (°C)
PRSS1 p.R122H	5′-GGT CCT GGG TCT CAT ACC TT-3′; 5′-GTA ATG GGC ACT CGA AAT GT-3′	60°
PRSS1 p.N29I	5′-CGC CAC CCC TAA CAT GCT AT-3′; 5′-CTC TCC CAG GCA GAC TGG CC-3′	64°
SPINK1 p.N34S	5′-CCA TTT CAG AGA TTT TGC TAT G-3′; 5′-GGT GAG ATT CAT ATT ATC AGT A-3′	60°
SERPINA1p. E366K	5′-AGC CTT ACA ACG TGT CTC TGC-3′; 5′-GGA TTT ACA GAT CAC ATG CAG G-3′	68°
SERPINA1p.E288V	5′-TCT TCC AAA CCT TCA CTC ACC-3′; 5′-GTC CCA ACA TGG CTA AGA GG-3′	61°