The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

Figure 5

Western blot and densitometry analysis of p-p38 (normalized to p38; (a)), p-ERK (normalized to ERK; (b)), and IκBα (normalized to tubulin; (c)) in activated macrophages lysates after incubation with 1 or 5 μM of P-5. C: naive control; U: untreated control. Shown are the mean ± S.D results. of 4–6 measurements. ANOVA of the effect of 1 and 5 μM of P-5 on the tested regulatory proteins were F_3,16 = 353.02 for p-38, F_3,20 = 47.19 for ERK, and F_3,16 = 97.01 for IκBα. ; ; compared to untreated cells.