The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

Moradov, Dorit; Shifrin, Helena; Harel, Efrat; Nadler-Milbauer, Mirela; Weinstock, Marta; Srebnik, Morris; Rubinstein, Abraham

doi:https://doi.org/10.1155/2015/939483

Gastroenterology Research and Practice

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Abstract Introduction Materials and Methods Results Discussion Conclusion Abbreviations Acknowledgments References Copyright Related Articles

Research Article | Open Access

Volume 2015 | Article ID 939483 | https://doi.org/10.1155/2015/939483

The Anti-Inflammatory Activity of a Novel Fused-Cyclopentenone Phosphonate and Its Potential in the Local Treatment of Experimental Colitis

Dorit Moradov,¹Helena Shifrin,¹Efrat Harel,¹Mirela Nadler-Milbauer,¹Marta Weinstock,¹Morris Srebnik,¹and Abraham Rubinstein^1,2,3

Academic Editor: Fabiana Zingone

Received04 Nov 2014

Revised15 Mar 2015

Accepted23 Mar 2015

Published09 Apr 2015

Abstract

A novel fused-cyclopentenone phosphonate compound, namely, diethyl 3-nonyl-5-oxo-3,5,6,6a-tetrahydro-1H-cyclopenta[c]furan-4-ylphosphonate (P-5), was prepared and tested in vitro (LPS-activated macrophages) for its cytotoxicity and anti-inflammatory activity and in vivo (DNBS induced rat model) for its potential to ameliorate induced colitis. Specifically, the competence of P-5 to reduce TNFα, IL-6, INFγ, MCP-1, IL-1α, MIP-1α, and RANTES in LPS-activated macrophages was measured. Experimental colitis was quantified in the rat model, macroscopically and by measuring the activity of tissue MPO and iNOS and levels of TNFα and IL-1β. It was found that P-5 decreased the levels of TNFα and the tested proinflammatory cytokines and chemokines in LPS-activated macrophages. In the colitis-induced rat model, P-5 was effective locally in reducing mucosal inflammation. This activity was equal to the activity of local treatment with 5-aminosalicylic acid. It is speculated that P-5 may be used for the local treatment of IBD (e.g., with the aid of colon-specific drug platforms). Its mode of action involves inhibition of the phosphorylation of MAPK ERK but not of p38 and had no effect on IκBα.

1. Introduction

Inflammatory bowel disease (IBD), a chronic inflammation of the gut, is characterized by a profound infiltration of macrophages and a continuous efflux of proinflammatory cytokines [1, 2] into the intestinal mucosa. The typical overproduction of tumor necrosis factor alpha (TNFα) in IBD has already led to the use of anti-TNFα monoclonal antibodies (e.g., infliximab) to block the access of TNFα to its receptor for the specific treatment of IBD [3, 4]. However, the use of these drugs is associated with severe adverse effects such as immunogenicity [5, 6], risk of lymphoma, and neuropathy [7, 8]. Thus, a rational approach to TNFα-based therapy may use inhibitors (e.g., metalloproteinase inhibitors) [9, 10] to prevent the TNFα-converting enzyme- (TACE-) mediated release of the soluble form of TNFα from its cell membrane-bound precursor into the vicinity of the inflamed regions [11, 12].

Phosphonate compounds possess a variety of pharmacological properties including antibacterial activity [13, 14] and nucleic acid or amino acid mimetic properties [15]. Phosphonate-phospholipid analogues can inhibit proinflammatory lipid mediators such as phospholipase A2 [16]. Phosphonate compounds are also efficient serine protease inhibitors [17]. They possess chelating properties [18] that enable them to inhibit the activity of zinc dependent enzymes such as matrix metalloproteinase and TACE [19–21]. They were also shown to ameliorate inflammation by reducing the activity of reactive oxygen species, as well as decreasing the levels of secreted TNFα [22–24].

Based on a synthetic method previously described by us [25], the goals of the present study were to (a) prepare the novel fused-cyclopentenone phosphonate, diethyl 3-nonyl-5-oxo-3,5,6,6a-tetrahydro-1H-cyclopenta[c]furan-4-ylphosphonate (denoted by P-5), (b) test whether P-5 can inhibit local secretion of TNFα, (c) examine, in vitro and in vivo, whether P-5 can ameliorate chronic inflammation, such as experimental colitis, and (d) explore mechanistically the possible anti-inflammatory effect of P-5.

2. Materials and Methods

2.1. Materials

Unless stated otherwise, all materials were purchased from Sigma (St. Louis, MO, Germany). Dimethyl sulfoxide (DMSO) was purchased from Merck (Darmstadt, Germany). Solvents were of analytical grade. Water was filtered and deionized by reverse osmosis (Barnstead Nanopure, Waltham, MA, USA). Thioglycollate broth was purchased from Difco (Lawrence KS, USA). Recombinant human TACE and the peptide Mca-P-L-A-Q-A-V-Dpa-R-S-S-S-R-NH₂ (Fluorogenic Peptide Substrate III) were purchased from R&D Systems, MN, USA. RIPA lysis buffer, containing a cocktail of phosphatases and proteases inhibitor (1%), was purchased from Bet Haemek, Israel.

Antibodies against IκBα, ERK, phosphorylated ERK (p-ERK), p38, phosphorylated p38 (p-p38), tubulin, iNOS, and GAPDH were purchased from Santa Cruz Biotechnologies, Santa Cruz, CA, USA. IRDye conjugated fluorescent secondary antibodies were as follows: donkey anti-mouse 680 was purchased from Rockland Immunochemicals, PA, USA; Alexa Fluor goat anti-rabbit 488 was purchased from Molecular Probes, NY, USA. TNFα and IL-6 ELISA kits were purchased from R&D Systems, MN, USA. IL-1β ELISA kit was purchased from PeproTech, Rocky Hill, USA. The IL-1α, INFγ, MCP-1, MIP-1α, and RANTES ELISA kit were purchased from Quansys biosciences, Utah, USA.

2.2. P-5 Preparation

Diethyl 3-nonyl-5-oxo-3,5,6,6a-tetrahydro-1H-cyclopenta[c]furan-4-ylphosphonate (P-5, Scheme 1) was synthesized by Pauson-Khand reaction from diethyl 3-(allyloxy)dodec-1-ynylphosphonate using Mo(CO)₆ and DMSO as described previously [25]. Briefly, to Mo(CO)₆ (1.2 eq) in dry toluene diethyl 3-(allyloxy)dodec-1-ynylphosphonate (1 eq) was added, followed by the addition of DMSO (5 eq). After refluxing for 6 h at 100°C the reaction mixture was cooled and ethyl acetate was added. The entire mixture was filtered through silica gel and the product was separated on silica gel column using gradient eluent of methanol/dichloromethane. The reaction yield was 58%.

2.3. Animals, Maintenance, and Euthanasia

C57BL/6 female mice (6–8 weeks old) and Sabra male rats (200–250 g), obtained from Harlan Laboratories, Jerusalem, Israel, were kept under constant environmental conditions (22°C, 12 h light/dark cycles) and fed with standard laboratory chow and tap water. All animal studies were conducted in accordance with the Principles of Laboratory Animal Care (NIH publication number 85-23, revised 1985). The joint Ethics Committee (IACUC) of the Hebrew University and Hadassah Medical Center approved the study protocol for animal welfare. The Hebrew University Animal Facility is an AAALAC international accredited institute (number 1285). Sedation of the mice was performed by isoflurane (USP, Terrell, Minrad Inc., USA). Euthanasia of the sedated mice was performed by cervical dislocation. Sedation of the rats was performed by intraperitoneal injection of a mixture of 100 mg/kg rat body weight of Ketamine (Ketaset, Fort Dodge, USA) and 2 mg per rat of Xylazine (Sedaxylan, Nederland). Euthanasia of the sedated rats was performed by puncture of the chest wall.

2.4. Peritoneal Macrophages: Induction and Harvesting

The mice were injected intraperitoneally with 1.5 mL of a 3% thioglycollate broth and sacrificed 4 days later. Immediately after the euthanasia, the recruited macrophages were aspirated from the inflamed tissue. The aspirated liquid containing cells was centrifuged; the suspended cells were plated in a 96-microwell flat-bottom plate (NUNC, Denmark) at a concentration of 1.5 × 10⁵ cells/well. After incubation, the medium was aspirated and the cells were rinsed with PBS to remove nonadherent cells.

2.5. P-5 Activity, Cytotoxicity, and TACE Inhibition Assessments

P-5 activity was tested towards activated macrophages. Elevated concentrations (1–20 μM) of P-5 in absolute ethanol, diluted with DMEM, were added to each well of a 96-microwell plate preseeded with the peritoneal macrophage cells (3–5 wells for each study). The macrophages were then activated by the addition of 25 μL of lipopolysaccharide (LPS, from Escherichia coli, serotype 0111:B4, Sigma Ltd.) to each well (final concentration of 1 μg/mL). The activated macrophages were then incubated and the supernatant fluid of each well was collected and kept frozen (−80°C) until analysis of TNFα. The steroid drug, budesonide, was used as a positive control at a final concentration of 10 μM. Wells containing LPS-activated cells without the addition of P-5 or budesonide served as negative (untreated) controls.

P-5 cytotoxicity was assessed by the MTT test. The 50% lethal concentration (LC₅₀) of P-5 was measured at a concentration range of 1–40 μM.

The possible inhibitory effect of P-5 on recombinant human TACE was assessed by incubating the enzyme (0.1 ng/mL) with increasing concentrations (1, 10 or 50 μM) of the compound in the presence of the fluorescent peptide substrate Mca-P-L-A-Q-A-V-Dpa-R-S-S-S-R-NH₂ [26]. The fluorescence which resulted by the TACE cleavage was measured at λex = 320 nm/λem = 405 nm. Doxycycline (100 μM) was used as a positive control in the inhibition study.

2.6. Cytokine Levels Determination

TNFα and IL-6 levels were measured by ELISA assay, employing a commercial kit (R&D Systems, MN, USA) according to the manufacturer’s instructions. IL-1α, INFγ, MCP-1, MIP-1α, and RANTES levels were analyzed by Q-Plex arrays, a multiplex commercial ELISA kit (Quansys biosciences, Utah, USA) according to the manufacturer’s instructions.

2.7. IκBα, p38, and ERK Analysis

Macrophage levels of IκBα, the protein kinase p38, and the extracellular signal-regulated kinase ERK were determined by Western blot. Isolated macrophages from the mice peritoneum were plated in 6-well culture plates (5 × 10⁶ cells/well). After 2-3 h, the incubation medium was aspirated and nonadherent cells were washed away with sterile PBS. P-5 (1 or 5 μM in a fresh complete DMEM medium) was then added. The control wells contained fresh medium only. Two hours later, the macrophages were activated with 5 μg/mL of LPS and further incubated (15 min) for IκBα analysis, 30 min for mitogen-activated protein kinases (MAPKs) phosphorylated p38, and 30 min for phosphorylated ERK analysis. The cells were then harvested and centrifuged and the cell pellets were shaken for 30 min, on ice, in a RIPA lysis buffer containing a cocktail of phosphatase and proteases inhibitors (1%). Protein concentration in the supernatant was determined by bicinchoninic acid protein assay kit (Thermo Scientific, USA). Protein samples (20 μg) were separated on 10% SDS polyacrylamide gels with 4.5% SDS stacking gel. Samples were electrotransferred onto nitrocellulose membranes (0.45 μm; Schleicher, Dassel, Germany). Blots were probed with antibodies against IκBα (1 : 500), ERK (1 : 300), phosphorylated ERK (p-ERK) (1 : 300), p38 (1 : 1000), phosphorylated p38 (p-p38) (1 : 600), and tubulin (1 : 1000). The nitrocellulose membranes were incubated with the appropriate primary antibodies and then incubated (1 h, room temperature) with appropriate IRDye conjugated fluorescent secondary antibodies: donkey anti-mouse 680 and Alexa Fluor goat anti-rabbit 488. IRDye conjugates are all optimized for the Odyssey Infrared Imaging System (LI-COR Biosciences, Lincoln, NE, USA). The densities of the obtained protein bands were quantified using TINA image analyzer software (version 2.07d; Raytest, Straubenhardt, Germany). The amount of IκBα was quantified and normalized to tubulin. The amount of p-p38 and p-ERK was quantified and normalized to band density of the nonphosphorylated entities, respectively.

2.8. Induction of Experimental Colitis in Rats and Treatment Protocol

The rats were deprived of food with free access to water 24 h prior to the colitis induction which was performed under light sedation (isoflurane inhalation) by intracolonic administration of 30 mg of DNBS in 1 mL of ethanol 25% (v/v) [27, 28]. One hour after the colitis induction P-5, at a dose of 10 mg/kg body weight, was administered intracolonically in 0.5 mL PBS containing 5% Tween 80 and 5% of absolute ethanol. The administration was repeated every 12 hours over a period of 3 days. Rats dosed with a 5-aminosalicylic acid (5-ASA) enema (268 mg/kg body weight) served as a positive control group [29]. A group of healthy rats was used as a naive control group. A group of untreated DNBS-induced rats served as a nontreated control group. On the fourth day, the rats were sacrificed and their colons exteriorized through a longitudinal abdominal incision.

2.9. Quantification of Inflammation Severity

The distal 10 cm of each colon was removed, cut open, and rinsed with ice-cold PBS, pH 7.4. Colon sections were blotted dry and weighed and the length was measured. Ulcerated and inflamed regions were identified. Scoring of the ulcerated areas was conducted by assigning 0.5 points for each 5 mm of ulcerated tissue [30].

2.10. Tissue Analysis of Inflammatory Markers

Tissue activity of myeloperoxidase (MPO) was analyzed in tissue homogenates (Polytron, Kinematiea GmbH, Germany) in 0.02 M phosphate-buffer, pH 7.4. After centrifugation and resuspension of the pellet in ice-cold phosphate buffer (50 mM, pH 6.0) containing 0.5% of hexadecyltrimethylammonium bromide (to release MPO from the primary granules of the neutrophils), the suspension was freeze-thawed, sonicated, and centrifuged. 10 μL of the supernatant was then added to 290 μL of phosphate buffer, containing o-dianisidine hydrochloride and hydrogen peroxide (5 × 10⁻⁴% v/v). The kinetics of absorbance change was measured at 460 nm over 30 sec. MPO activity was calculated using a 6-point calibration curve employing purified peroxidase [31]. MPO activity (per μg total tissue protein) was expressed as a fraction (in %) of the enzyme activity normalized to the tissue activity in the untreated control group.

TNFα and IL-1β levels in the homogenized colon tissues were measured using ELISA kits. After centrifugation, the separated supernatant was poured into a 96-well MaxiSorb ELISA plate and processed according to the manufacturer’s protocol. Cytokine levels (pg range) were expressed as a fraction (in %) of cytokine concentration in the colon tissues and were normalized to both total tissue protein and cytokine level in the untreated control group.

Inducible nitric oxide synthase (iNOS) activity was determined by immunoblotting as described above with relevant modifications. Colon tissues were homogenized in RIPA lysis buffer. The samples were blotted with antibodies against NOS2 (1 : 500) and GAPDH (1 : 1000). Tissue amount of iNOS was normalized to GAPDH levels.

Protein tissue levels were measured by the Bradford method [32] to allow normalizing of MPO activity and levels of TNFα and IL-1β to total tissue protein (pg/μg tissue).

2.11. Statistical Analysis

The results are expressed as means ± S.D. values. Differences between data obtained from cells treated with LPS and cells treated with LPS plus P-5 at various concentrations and differences between data obtained from DNBS-induced rats with and without P-5 or 5-ASA were analyzed by ANOVA using IBM SPSS Statistics Ver. 19, followed by Duncan’s post hoc test. A value of <0.05 was considered to be significant.

3. Results

The cytotoxicity of the fused-cyclopentenone phosphonate compound P-5 is shown in Figure 1, which demonstrates that its LC₅₀ is 20 μM and that, in concentration of 5 μM or less, P-5 did not show any cytotoxicity towards the cells. The effect of P-5 on TNFα levels in the peritoneal macrophages was measured and its IC₅₀ was identified as 6.1 μM. Figure 2 shows that 10 μM of P-5 caused a reduction of 80% in TNFα secretion. At this dose, the viability of the cells was found to be 60% (Figure 1) which may be the cause for the profound reduction in TNFα levels. However, at a concentration of 5 μM which showed no cytotoxicity toward the cells (Figure 1), there was a reduction of about 50% in TNFα levels (Figure 2). Figure 2 also shows that P-5 reduced TNFα levels in the peritoneal macrophages in a dose-dependent manner.

To elucidate whether the reduction in TNFα levels caused by P-5 was a result of TACE inhibition, increasing amounts of the compound were incubated with the fluorescently tagged TACE peptide substrate Mca-P-L-A-Q-A-V-Dpa-R-S-S-S-R-NH₂ in the presence of purified human recombinant TACE. A similar study with 100 μM of doxycycline served as a positive control. The results shown in Figure 3 demonstrate that TACE inhibition was not the cause for P-5 activity.

Figure 4 shows how P-5 attenuated the secretion of a series of cytokines and chemokines in a dose dependent manner (dose range of 1–20 μM) in the LPS-activated macrophages. While P-5 reduced IL-6, INFγ, and MCP-1 levels at its lowest concentration (1 μM; Figures 4(a), 4(c), and 4(d)), higher doses were required for reducing the levels of IL-1α (2.5 μM; Figure 4(b)), MIP-1α, and RANTES (10 μM; Figures 4(e) and 4(f)). Since P-5 was not associated with TACE inhibition, its effect on p38, ERK, and IκBα levels was also queried. The Western bolt analysis of p-p38, p-ERK, and IκBα shows that while P-5 did not affect p38 and IκBα levels, it significantly reduced the phosphorylation of ERK (Figure 5).

(a)

(b)

(c)

(d)

(e)

(f)

Figure 4

The attenuating effect of P-5 on the secretion of IL-6, IL-1α, INFγ, MCP-1, MIP-1α, and RANTES in LPS-activated macrophages. Results are expressed as the fraction (in %) of the cytokine level secreted by untreated activated cells. N: naive control; U: untreated control. Shown are the mean results ± S.D. ( = 3-4). ANOVA of the effect of P-5 at a series of concentrations on IL-6 levels was F_7,33 = 115.36, , on IL-1α was F_6,20 = 8.06, , on INFγ was F_6,19 = 4.64, , on MCP-1 was F_6,20 = 73.57, , on MIP-1α was F_7,23 = 219.00, , and on RANTES was F_5,17 = 100.54, . ; ; compared to untreated cells.

Figure 5

Western blot and densitometry analysis of p-p38 (normalized to p38; (a)), p-ERK (normalized to ERK; (b)), and IκBα (normalized to tubulin; (c)) in activated macrophages lysates after incubation with 1 or 5 μM of P-5. C: naive control; U: untreated control. Shown are the mean ± S.D results. of 4–6 measurements. ANOVA of the effect of 1 and 5 μM of P-5 on the tested regulatory proteins were F_3,16 = 353.02 for p-38, F_3,20 = 47.19 for ERK, and F_3,16 = 97.01 for IκBα. ; ; compared to untreated cells.

The local anti-inflammatory activity of P-5 as assessed, macroscopically, in the colon of DNBS-induced rats is summarized in Table 1, which shows that, after rectal administration of P-5, the weight of the inflamed colons was reduced by 33% (43% reduced by 5-ASA, the positive control). Ulceration scoring revealed that both P-5 and 5-ASA treatments reduced the severity of ulceration.

Quantification of the severity of inflammation was conducted by measuring tissue MPO activity, iNOS expression, and TNFα and IL-1β levels. Figure 6 shows that MPO activity was increased by 80% in the colon tissues of the DNBS-induced rats, untreated group. Local treatment with P-5 attenuated the enzyme activity almost back to normal, while 5-ASA reduced the activity by 56%. P-5 reduced iNOS expression by 78% compared to the DNBS-induced group and decreased the levels of TNFα and IL-1β by 50 and 68%, respectively. The reduction caused by 5-ASA treatment was 67 and 69%, respectively.

(a)

(b)

(c)

(d)

Figure 6

MPO activity (a), iNOS expression (b), TNFα (c), and IL-1β (d) tissue levels in the colons of healthy rats, untreated DNBS-induced rats, and DNBS-induced rats treated with P-5 and 5-ASA. Results are normalized to total tissue protein. (a), (c), and (d) are expressed as a fraction (in %) of the tissue biomarker levels in the DNBS-treated group. (b) is normalized to the house keeping gene, GAPDH. Shown are the mean ± S.D. values of 3–7 rats in each group. ANOVA of the effect of P-5 on MPO activity was F_3,19 = 13.63 , on iNOS expression was F_2,18 = 37.57 , on TNFα level was F_3,22 = 9.00 , and on IL-1β level was F_3,31 = 22.61 . ; ; compared with the DNBS group.

4. Discussion

Our study shows that diethyl 3-nonyl-5-oxo-3,5,6,6a-tetrahydro-1H-cyclopenta[c]furan-4-ylphosphonate (P-5) (Scheme 1), a vinylphosphonate compound, containing a furan ring fused to a cyclopentenone ring, showed profound anti-inflammatory activity, in LPS-activated macrophage cells and in colitis-induced rat model. Cyclopentenone compounds were already tested with respect to IBD. For example, Cuzzocrea and coworkers showed that the reactive α,β-unsaturated carbonyl group, located in the cyclopentenone ring can ameliorate proinflammatory activity by reducing the activation of the nuclear factor kappa light chain enhancer of activated B (NF-κB) cells [33]. The LC₅₀ of P-5 was 15–20 μM (Figure 1). Its IC₅₀ in LPS-activated macrophages was calculated to be 6.1 μM, a concentration in which P-5 was not cytotoxic. A lower concentration (5 μM) also showed a profound reduction in TNFα levels. Because phosphonates are potential zinc-dependent metalloproteinase inhibitors [21, 34], we examined the possible inhibitory effect of P-5 on purified human recombinant TACE [35, 36] and found no effect (Figure 3).

Although TNFα is a major player in chronic inflammation and its amelioration [4, 37], other mediators, cytokines [38, 39], and chemokines [40–42] are involved in the inflammatory process. Consequently, the effect of P-5 in attenuating IL-6, IL-1α, INF-γ, MCP-1, MIP-1α, and RANTES in activated macrophages was tested in the concentration range of 1–20 μM. The results shown in Figure 4 demonstrate that the compound possessed a profound anti-inflammatory effect, which did not involve TACE inhibition (Figure 3). An alternative mode of action could be an involvement in one or more signaling transduction pathways that are activated by LPS and/or the inflammation cascade. Indeed, the last part of the study explored the possible effect of P-5 on the activity of extracellular signal-regulated kinases (ERK) [43] and p38 [44] of the MAPK inflammation mediator family [45], as well as its possible involvement in the NF-κB pathway [46, 47]. As demonstrated in Figure 5, P-5 did not prevent the degradation of IκBα, which may indicate that P-5 did not affect NF-κB. Also, P-5 did not interfere with the phosphorylation of p38; however, it did reduce the phosphorylation of ERK at two concentrations, 1 and 5 μM.

In the last step of the study, the anti-inflammatory effect of P-5 was verified in vivo in the DNBS-induced rat model. A preliminary study was conducted in an attempt to identify the optimal effective dose in which P-5 reduces inflammation, when it is administrated intracolonically. The concentration was found to be 10 mg/kg body weight (data not shown). This dose of P-5 or 5-ASA (268 mg/kg body weight) as a positive control was administered rectally twice daily for three days. The macroscopic analysis shown in Table 1 demonstrates that the anti-inflammatory effect of P-5 was similar to that of 5-ASA. The biochemical analysis performed on the rat colon specimens confirmed the assumption that P-5 could serve as a local therapeutic agent in the treatment of IBD. In addition to reducing MPO activity to values similar to those in healthy colon, it reduced the mucosal levels of TNFα and IL-1β (Figure 6), a pattern akin to what was observed in the LPS-activated macrophages. P-5 administration also decreased iNOS expression in the treated colons, which could have led to reduced tissue NO levels. All this suggested that P-5 activity may involve amelioration of inflammation-driven oxidative stress [48]. This antioxidant activity of P-5 could result from its enone moiety in the cyclopentenone ring. The electrophilic nature of the α,β-unsaturated ketone can readily interact with reactive oxygen species, thus leading to termination of a free radical chain reaction occurring under oxidative conditions [49].

5. Conclusion

The fused-cyclopentenone phosphonate compound, P-5, possesses TACE-independent anti-inflammatory activity. Its mode of action involves reduction of the phosphorylation of ERK but it does not affect p38 or IκBα and, hence, it has no effect on the expression of NF-κB transcription factor. We speculate that P-5 may serve as an anti-inflammatory agent in the local treatment of colitis; however, human studies substantiation is required.

Abbreviations

DNBS:	2,4-Dinitrobenzene sulfonic acid
INFγ:	Interferon γ
IBD:	Inflammatory bowel disease
IL:	Interleukin
iNOS:	Inducible nitric oxide synthase
LPS:	Lipopolysaccharide
MCP-1:	Monocyte chemotactic protein-1
MIP-1α:	Macrophage inflammatory protein 1α
MPO:	Myeloperoxidase
RANTES:	Regulated on activation normal T cell expressed and secreted
TACE:	TNFα-converting enzyme
TNFα:	Tumor necrosis factor alpha.

Conflict of Interests

The authors declare no competing financial interests.

Acknowledgments

The work was supported by the Chief Scientist Office of the Israeli Ministry of Health (Grant no. 01797). It was included in the Ph.D. thesis of Dorit Moradov as a partial requirement of The Hebrew University of Jerusalem.

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Copyright © 2015 Dorit Moradov et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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