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Gastroenterology Research and Practice
Volume 2016, Article ID 1738430, 6 pages
http://dx.doi.org/10.1155/2016/1738430
Research Article

The Effects of Infliximab on Laminin, NFκB, and Anti-TNF Expression through Its Effect on Ischemic Liver Tissue

1School of Medicine, Department of Gastroenterology, Recep Tayyip Erdoğan University, 53100 Rize, Turkey
2School of Medicine, Department of Embriology and Histology, Recep Tayyip Erdoğan University, 53100 Rize, Turkey
3School of Medicine, Department of Hematology, Recep Tayyip Erdoğan University, 53100 Rize, Turkey

Received 22 February 2016; Accepted 22 March 2016

Academic Editor: Paolo Gionchetti

Copyright © 2016 Remzi Adnan Akdogan et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to investigate the possible protective effects of infliximab on expression of laminin, anti-TNF, and NFκB in the rat hepatic cells after ischemia/reperfusion (I/R). A total of 30 male Wistar albino rats were divided into three groups: Control (C), sham I/R (ISC), and I/R+ infliximab (ISC inf); each group comprised 10 animals. C group animals underwent laparotomy without I/R injury. In ISC groups after undergoing laparotomy, 1 hour of superior mesenteric artery ligation was done, which was followed by 1 hour of reperfusion. In the ISC inf group, 3 days before I/R, infliximab (3 mg/kg) was administered intravenously. All animals were killed at the end of reperfusion and hepatic tissue samples were obtained for histopathological and histochemical investigations in all groups. Laminin, anti-TNF, and NFκB immunoreactivity were performed for all groups. ISC caused severe histopathological injury including mucosal erosions, inflammatory cell infiltration, necrosis, hemorrhage, and villous congestion. Infliximab treatment significantly attenuated the severity of intestinal I/R injury and it is shown by laminin, anti-TNF, and NFκB immunoreactivity. Because of its anti-inflammatory and antioxidant effects, infliximab pretreatment may have protective effects on hepatic cells in the experimental intestinal I/R model of rats.