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Gastroenterology Research and Practice
Volume 2016 (2016), Article ID 2540397, 6 pages
http://dx.doi.org/10.1155/2016/2540397
Research Article

Postcolectomy Peritoneal Environment Increases Colon Cancer Cell Migration Capacity

1Department of Surgery B, Meir Medical Center, 44281 Kfar Saba, Israel
2Sackler Faculty of Medicine, Tel-Aviv University, 6997801 Tel-Aviv, Israel
3Department of Oncology, Meir Medical Center, 44281 Kfar Saba, Israel

Received 6 June 2015; Revised 24 September 2015; Accepted 28 September 2015

Academic Editor: Antoni Castells

Copyright © 2016 Liron Berkovich et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. Clinical data and animal models support an association between postoperative inflammatory response and the risk of colorectal cancer recurrence. Our aim was to evaluate postoperative peritoneal inflammation and its impact on cultured colon cancer cells’ migration capacity. Methods. 23 patients undergoing elective colorectal resection with uneventful recovery were prospectively enrolled. Patients were operated on for both malignant and benign etiologies. Peritoneal fluids collected at surgery initiation and after surgery were evaluated for their effect on migration potential of human colon cancer cells using an in vitro scratch assay and on TNF-α, IL-1β, IL-6, and IL-10 levels using bead-based fluorokine-linked multianalyte profiling. Results. Postoperative peritoneal fluid from all patients increased the migration capacity of colon cancer cells compared to preoperative levels. This effect was significant during the first two postoperative days and decreased thereafter. The increase in colon cancer cell migration capacity correlated with increased levels of peritoneal TNF-α and IL-10. Conclusion. In this pilot study, we have demonstrated that the intraperitoneal environment following colorectal resection significantly enhances colon cancer cells migration capacity. This effect is associated with postoperative intra-abdominal cytokines level. A larger scale study in colorectal cancer patients is needed in order to correlate these findings with perioperative parameters and clinical outcome.