Treatment with a Monoclonal Anti-IL-12p40 Antibody Induces Substantial Gut Microbiota Changes in an Experimental Colitis Model

Figure 1

Induction of AdTr-colitis is associated with weight loss, loose stools, and colonic inflammation. (a) Delta weight loss from day 21 is shown as mean ± SEM for individual groups. Significant differences between the areas under the curve (AUC) for individual mice were determined by Student’s -test. (b) DAI was determined with a combined scale from 0 to 8 by combining weight loss (0–4) and stool score (0–4). Differences in DAI scores were calculated as AUC for individual mice from 21 to 45 days using Mann-Whitney test. (c) The colon weight- (mg) to-length (cm) ratio was calculated for individual mice. Comparisons between groups were performed using Student’s -test. (d) Endoscopic analysis of colon at 21 and 34 days was graded with MEICS. All mice were scored semiquantitatively in a blinded fashion. Significant differences were determined with Mann-Whitney test for unpaired analysis and Wilcoxon for paired analysis.

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