Suppressing Syndecan-1 Shedding Ameliorates Intestinal Epithelial Inflammation through Inhibiting NF-κB Pathway and TNF-α
SDC1 shedding is blocked in mut-SDC1 transfected IEC-6 cells. PMA was used to induce SDC1 shedding in IEC-6 cells after being transfected with wt-SDC1 or mut-SDC1 plasmid. (a) Protein levels and mRNA levels of SDC1 in the cell were measured by western blot and RT-PCR; GAPDH was used as the loading control (left). Protein levels of cell surface SDC1 were measured by immunofluorescence. SDC1 (red) and cell nucleus (blue). Original magnifications: 400x. (b) Levels of shed SDC1 in the cell culture supernatant were detected by ELISA (upper) and dot blot (lower) ().
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