TY - JOUR A2 - Plevris, John N. AU - Zhu, Xueping AU - Xiao, Zhihui AU - Xu, Yumin AU - Zhao, Xingli AU - Cheng, Ping AU - Cui, Ningxun AU - Cui, Mingling AU - Li, Jie AU - Zhu, Xiaoli PY - 2016 DA - 2016/01/05 TI - Differential Impacts of Soybean and Fish Oils on Hepatocyte Lipid Droplet Accumulation and Endoplasmic Reticulum Stress in Primary Rabbit Hepatocytes SP - 9717014 VL - 2016 AB - Parenteral nutrition-associated liver disease (PNALD) is a severe ailment associated with long-term parenteral nutrition. Soybean oil-based lipid emulsions (SOLE) are thought to promote PNALD development, whereas fish oil-based lipid emulsions (FOLE) are thought to protect against PNALD. This study aimed to investigate the effects of SOLE and FOLE on primary rabbit hepatocytes. The results reveal that SOLE caused significant endoplasmic reticulum (ER) and mitochondrial damage, ultimately resulting in lipid droplets accumulation and ER stress. While these deleterious events induce hepatocyte injury, FOLE at high doses cause only minor ER and mitochondrial damage, which has no effect on hepatic function. SOLE also significantly upregulated glucose-regulated protein 94 mRNA and protein expression. These data indicate that SOLE, but not FOLE, damage the ER and mitochondria, resulting in lipid droplets accumulation and ER stress and, finally, hepatocyte injury. This likely contributes to the differential impacts of SOLE and FOLE on PNALD development and progression. SN - 1687-6121 UR - https://doi.org/10.1155/2016/9717014 DO - 10.1155/2016/9717014 JF - Gastroenterology Research and Practice PB - Hindawi Publishing Corporation KW - ER -