TY - JOUR
A2 - Silvestris, Nicola
AU - Zhang, Tao
AU - Liu, Chang
AU - Huang, Shi
AU - Ma, Yuanping
AU - Fang, Jiansong
AU - Chen, Yuanneng
PY - 2017
DA - 2017/05/04
TI - A Downmodulated MicroRNA Profiling in Patients with Gastric Cancer
SP - 1526981
VL - 2017
AB - Objective. Here, we aim to investigate the microRNA (miR) profiling in human gastric cancer (GC). Methods. Tumoral and matched peritumoral gastric specimens were collected from 12 GC patients who underwent routine surgery. A high-throughput miR sequencing method was applied to detect the aberrantly expressed miRs in a subset of 6 paired samples. The stem-loop quantitative real-time polymerase chain reaction (qRT-PCR) assay was subsequently performed to confirm the sequencing results in the remaining 6 paired samples. The profiling results were also validated in vitro in three human GC cell lines (BGC-823, MGC-803, and GTL-16) and a normal gastric epithelial cell line (GES-1). Results. The miR sequencing approach detected 5 differentially expressed miRs, hsa-miR-132-3p, hsa-miR-155-5p, hsa-miR-19b-3p, hsa-miR-204-5p, and hsa-miR-30a-3p, which were significantly downmodulated between the tumoral and peritumoral GC tissues. Most of the results were further confirmed by qRT-PCR, while no change was observed for hsa-miR-30a-3p. The in vitro finding also agreed with the results of both miR sequencing and qRT-PCR for hsa-miR-204-5p, hsa-miR-155-5p, and hsa-miR-132-3p. Conclusion. Together, our findings may serve to identify new molecular alterations as well as to enrich the miR profiling in human GC.
SN - 1687-6121
UR - https://doi.org/10.1155/2017/1526981
DO - 10.1155/2017/1526981
JF - Gastroenterology Research and Practice
PB - Hindawi
KW -
ER -