The Methylation Status and Expression of Epstein-Barr Virus Early Genes BARF1 and BHRF1 in Epstein-Barr Virus-Associated Gastric Carcinomas

Figure 3

The methylation status of BARF1 and BHRF1 promoters in gastric carcinoma tissue by MSP and BSP. (a, c) The methylation status of BARF1 and BHRF1 promoters in gastric carcinoma tissue by MSP. M, methylated; U, unmethylated. (b) The methylation status of the 2 early gene promoters in gastric carcinoma tissue by BS. “Black circle” indicates methylated CpG site; “white circle” indicates unmethylated CpG site. The CpG sites in BSP region of BARF1and BHRF1 were 50 and 26. (d) Sequencing result of BARF1 gene promoter in EBV-positive gastric carcinoma cell line named GT38. The 8 CpG sites in the sequence correspond to 32–40 CpG sites in Figure 2(a).

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