Research Article
Fucosterol Protects against Concanavalin A-Induced Acute Liver Injury: Focus on P38 MAPK/NF-κB Pathway Activity
Figure 3
Effects of fucosterol on autophagy during acute liver injury. (a) The expression of Beclin-1, LC3-II, and P62 mRNA were evaluated by real-time PCR. (b) Western blot and analysis of Beclin-1, LC3-II, and P62 proteins. (c) Immunohistochemistry was used to detect the level of Beclin-1, LC3-II, and P62 expression at 8 h. Original magnification: 200x. (d) Autophagosome formation was detected in liver tissues with transmission electron microscopy at 8 h (magnification, ×10,000). Arrows indicate autophagosomes. Data are expressed as mean ± SD (, for ConA versus control, for ConA + fucosterol (25 mg/kg) versus ConA, for ConA + fucosterol (50 mg/kg) versus ConA + fucosterol (25 mg/kg), and for ConA + fucosterol (100 mg/kg) versus ConA + fucosterol (50 mg/kg)).
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