Fucosterol Protects against Concanavalin A-Induced Acute Liver Injury: Focus on P38 MAPK/NF-κB Pathway Activity

Figure 5

Effect of fucosterol on MAPK family activation. (a) Western blot and analysis of P38, p-P38, JNK, p-JNK, ERK, and p-ERK. (b) Western blot and analysis of p-MKK3/6, MKK3 and MKK6. (c) Immunohistochemistry was used to detect p-P38 and p-MKK3/6 levels at 8 h. Original magnification: 200x. (d) The proliferation of LO2 with CCK8 assay and Western blot and analysis of P38, p-P38, PPARγ, and RXRα. Data are expressed as mean ± SD (, for ConA versus control, for ConA + fucosterol (25 mg/kg) versus ConA, for ConA + fucosterol (50 mg/kg) versus ConA + fucosterol (25 mg/kg), and for ConA + fucosterol (100 mg/kg) versus ConA + fucosterol (50 mg/kg)).

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