DAB2IP Downregulation Enhances the Proliferation and Metastasis of Human Gastric Cancer Cells by Derepressing the ERK1/2 Pathway

Figure 4

Downregulation of DAB2IP expression promotes EMT at both protein and mRNA levels in gastric cancer cells. (a) Western blotting analysis of the E-cadherin and vimentin expression in wild-type AGS cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). Tubulin as a loading control. (b) The bands were quantified and presented as the mean ± SEM of three independent experiments. (c) QRT-PCR analysis of the E-cadherin and vimentin mRNA expression in wild-type AGS cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). The bands were presented as the mean ± SEM (). (D) Western blotting analysis of the E-cadherin and vimentin expression in wild-type SGC7901 cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). Tubulin as a loading control. (e) The bands were quantified and presented as the mean ± SEM of three independent experiments. (f) QRT-PCR analysis of the E-cadherin and vimentin mRNA expression in wild-type SGC7901 cells (shControl) and in cells with stable knockdown of DAB2IP (shDAB2IP). The bands were presented as the mean ± SEM (). Statistical significance was determined by a two-tailed, unpaired Student t-test. ; ; .

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