Differential Effects of Three Techniques for Hepatic Vascular Exclusion during Resection for Liver Cirrhosis on Hepatic Ischemia-Reperfusion Injury in Rats

Figure 5

Effects of different techniques for hepatic vascular exclusion during resection on TLR4 protein levels after hepatic I/R in rats. WB analysis was performed to determine protein levels of TLR-4 and GAPDH in the rat liver tissues. The levels of TLR4 protein were normalized to GAPDH levels as described in the Materials and Methods. (a) WB analysis of TLR4 protein levels at different time points (1, 3, 6, and 24 h) after reperfusion in the PR, HH, and WH groups; (b) normalized TLR4 protein levels at different time points (1, 3, 6, and 24 h) after reperfusion in the PR, HH, and WH groups. TLR4 protein levels in the PR group were significantly higher than those in the HH and WH groups (), whereas there was no significant difference between the HH and WH groups.

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