DNMT3B Expression Might Contribute to Abnormal Methylation of RASSF1A in Lager Colorectal Adenomatous Polyps

Figure 3

Global methylation of LINE-1 and Alu in CAP and NCM tissues. (a) Representative electrophoresis images of COBRA products (LINE-1 DNA sequence + Taq1 digestion). (b) The statistical analysis showed the rate of DNA methylation in the LINE-1 DNA sequence has a difference between CAP and control tissues(). (c) Representative electrophoresis images of COBRA products (LINE-1 DNA sequence + TSP509I digestion) in agarose gel. (d) The statistical analysis showed the rate of DNA unmethylation in the LINE-1 DNA sequence has a difference between CAP and control tissues(). (e) Representative electrophoresis images of COBRA products (Alu DNA sequence + Mbo1 digestion). (f) The statistical analysis showed the rate of DNA methylation in Alu DNA sequence did not exhibit any difference between CAP and control tissues.

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