Research Article

Silencing of Long Noncoding RNA LINC00324 Interacts with MicroRNA-3200-5p to Attenuate the Tumorigenesis of Gastric Cancer via Regulating BCAT1

Figure 4

LINC00324 positively regulated BCAT1 expression via binding to miR-3200-5p. (a) TargetScan predicted a binding site between BCAT1 and miR-3200-5p. (b) Relative luciferase activity in MGC803 and MKN-45 cells was measured by the dual-luciferase reporter assay. , vs. mimics NC; # vs. miR-3200-5p mimics. (c) The interaction between BCAT1 and miR-3200-5p in MGC803 and MKN-45 cells was detected by the RNA pull-down assay. vs. Bio-NC. (d) The expression of BCAT1 in MGC803 and MKN-45 cells was detected by qRT-PCR. vs. si-NC+inhibitor NC and si-LINC00324+miR-3200-5p inhibitor. (e) The expression of BCAT1 in tumor tissues and adjacent normal tissues of patients with GC was detected by qRT-PCR. vs. normal tissue. (f) The expression of BCAT1 was negatively correlated with miR-3200-5p. (g) The expression of BCAT1 was positively correlated with LINC00324.
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