Immune Modulation Effects of Lactobacillus casei Variety rhamnosus on Enterocytes and Intestinal Stem Cells in a 5-FU-Induced Mucositis Mouse Model

Figure 4

Effect of Lcr35 administration on the T lymphocyte count by flow cytometry analysis. (a) A tremendous rise of the CD3⁺/CD8⁺ lymphocyte count in the 5-FU group ( K/μl) was observed when compared to that in the saline group ( K/μl). The CD8 T lymphocyte count in the 5-FU+Lcr35 group ( K/μl) was significantly lower than that in the 5-FU group (). (b) A significant increase of the CD3⁺/CD4⁺ T lymphocyte count was found in the 5-FU group ( K/μl) when compared to the saline group ( K/μl). The CD3⁺/CD4⁺ T lymphocyte count in the 5-FU+Lcr35 group ( K/μl) was lower than that in the 5-FU group though no significant difference was found (). (c) The CD4⁺/IL-4 T lymphocyte count in the 5-FU+Lcr35 group ( K/μl) was significantly higher than that in the 5-FU group ( K/μl) (). (d) A tremendous rise of the CD4+/IL-17A lymphocyte count in the 5-FU group was observed when compared to that in the saline group. The CD4⁺/IL-17A T lymphocyte count in the 5-FU+Lcr35 group ( K/μl) was significantly higher than that in the 5-FU group ( K/μl) (). Statistical analysis was performed by one-way ANOVA.

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