Table of Contents
Hepatitis Research and Treatment
Volume 2010, Article ID 429243, 10 pages
http://dx.doi.org/10.1155/2010/429243
Clinical Study

Tumor Necrosis Factor Receptor 1 Expression Is Upregulated in Dendritic Cells in Patients with Chronic HCV Who Respond to Therapy

1Division of Digestive and Liver Diseases, Department of Internal Medicine, Southwestern Medical Center at Dallas, University of Texas at Dallas, Dallas, TX 75390-9151, USA
2Dallas Veterans Affairs Medical Center, 4500 S. Lancaster Road, Dallas, Texas 75216, USA

Received 25 March 2010; Revised 28 May 2010; Accepted 31 May 2010

Academic Editor: Tatehiro Kagawa

Copyright © 2010 Raul Cubillas et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The present studies assessed the level of tumor necrosis factor receptor (TNFR) expression in peripheral blood mononuclear cells (PBMCs) subsets from patients with chronic HCV undergoing interferon š›¼ /ribavirin-based therapy (Ifn/R). Methods. TNFR family member mRNA expression was determined using quantitative real-time PCR assays (RTPCRs) in PBMC from 39 HCV+ patients and 21 control HCV āˆ’ patients. Further subset analysis of HCV + patients (untreated (U), sustained virological responders (SVR), and nonresponders (NR)/relapsers (Rel)) PBMC was performed via staining with anti-CD123, anti-CD33, anti-TNFR1 or via RTPCR for TNFR1 mRNA. Results. A similar level of TNFR1 mRNA in PBMC from untreated HCV+ genotype 1 patients and controls was noted. TNFR1 and TNFR2 mRNA levels in PBMC from HCV+ patients with SVR were statistically different than levels in HCV( āˆ’ ) patients. A significant difference was noted between the peak values of TNFR1 of the CD123+ PBMC isolated from SVR and the NR/Rel. Conclusion. Upregulation of TNFR1 expression, occurring in a specific subset of CD123+ dendritic cells, appeared in HCV+ patients with SVR.