HD-03/ES: A Herbal Medicine Inhibits Hepatitis B Surface Antigen Secretion in Transfected Human Hepatocarcinoma PLC/PRF/5 Cells
Figure 3
The effect of HD-03/ES on HBsAg gene expression. PLC/PRF/5 cells were treated with or without HD-03/ES at three nontoxic concentrations. RNA was isolated from drug-treated and -untreated cells and multiplex RT-PCR was performed using specific primers as described in the text. (a) RT-PCR product of S-gene and GAPDH resolved in 2% agarose gel. (b) Densitometric analysis of the gene transcripts and the values depict arbitrary units. Data is representative of two experiments.
