MHC Class I Presented T Cell Epitopes as Potential Antigens for Therapeutic Vaccine against HBV Chronic Infection
HBV peptide specific CD8+ T cells activated in vivo secrete cytotoxic effector molecules. In an assay that mirrored the setup described in Figure 5, splenocytes were cultured with peptide pulsed (peptides are represented as first 3 residues of the sequence) or HBV expressing cells overnight. Supernatant was harvested and used in the Milliplex magnetic bead assay to detect granzyme B secretion in response to specific stimulation. Splenocytes from PBS primed, naïve mice were used as a negative control.
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