A Novel Structurally Stable Multiepitope Protein for Detection of HCV

Figure 3

Specificity test for rMEHCV. (a) Detection of HCV using an in-house EIA. Each well of the EIA plates was coated with 20 ng purified rMEHCV and assayed with 17 positive and 10 negative human sera samples. The white bar represents the control “blank” test (0 ng protein coated + serum # 1 + conjugated secondary antibody), and the black bars and gray bars represent the sera positive and negative for anti-HCV, respectively. The bars represent the average of triplicates of each serum with its respective standard deviation. (b) Analysis of samples tested in (a) with a commercial kit (Hepanóstika HCV Ultra kit). The sera were diluted 1/10.

(a)

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