Research Article

A Novel Structurally Stable Multiepitope Protein for Detection of HCV

Figure 4

Cross-reactivity test of rMEHCV using in-house EIA. Human sera samples were diluted 1/20 and secondary antibody was diluted 1/25,000. The bars represent the standard deviation of triplicates. The white bar represents the control “blank” test (0 ng protein coated + serum # 1 + conjugated secondary antibody), and the black, gray, and crosshatched bars represent positive sera, negative for anti-HCV and positive for other diseases, respectively. Sample numbering follows the list shown in Table 1.