A Novel Structurally Stable Multiepitope Protein for Detection of HCV

Figure 6

Heat-induced unfolding curve of rMEHCV (2.5 μM) monitored by CD spectroscopy at 208 nm in 5 mM Tris-HCl pH 7.0. The black line corresponds to the sigmoid fitting of experimental data. The fraction unfolded data are calculated considering the changes in molar ellipticities at 208 nm and equations described in Material and Methods. The melting temperature, , corresponding to the inflection point of the sigmoid is 66.32°C.