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HPB Surgery
Volume 2011 (2011), Article ID 789323, 9 pages
http://dx.doi.org/10.1155/2011/789323
Research Article

Disturbances in the Glutathione/Ophthalmate Redox Buffer System in the Woodchuck Model of Hepatitis Virus-Induced Hepatocellular Carcinoma

1Department of Surgery, Case Western Reserve University School of Medicine and University Hospitals, Case Medical Center, Cleveland, OH 44106-7029, USA
2Department of Nutrition, Case Western Reserve University School of Medicine and University Hospitals, Case Medical Center, Cleveland, OH 44106-7029, USA
3Department of Pathology, Case Western Reserve University School of Medicine and University Hospitals, Case Medical Center, Cleveland, OH 44106-7029, USA
4Department of Radiology, Case Western Reserve University School of Medicine and University Hospitals, Case Medical Center, Cleveland, OH 44106-7029, USA
5Division of Transplant and Hepatobiliary Surgery, Department of Surgery, University Hospitals, Case Medical Center, Lakeside 7510, PS 5047, Cleveland, OH 44106, USA

Received 15 May 2011; Revised 18 July 2011; Accepted 19 July 2011

Academic Editor: Vito R. Cicinnati

Copyright © 2011 Rafael Andres Ibarra et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Purpose. The incidence of liver tumors is rising in USA. The purpose of this study was to evaluate liver oxido-reductive status in the presence of chronic liver disease and hepatocellular carcinoma (HCC). Methods. Glutathione species and ophthalmate (OA) concentrations were measured by LC-MS in processed plasma and red blood cells (RBC) from infected Woodchuck with hepatitis virus (WHV). Blood samples were obtained from: (i) infected animals with tumors (WHV+/HCC+), (ii) infected animals without tumors (WHV+/HCC−) and (iii) healthy animals (WHC−/HCC−). Results. The concentration of reduced glutathione (GSH) and the ratio GSH/GSG were lower in plasma from WHV+/HCC+ animals when compared to WHV+/HCC− and WHV−/HCC− (P<0.01). In contrast, the concentration of oxidized glutathione (GSSG) was found to be higher in plasma from WHV+/HCC+ animals when compared to WHV+/HCC− and WHV−/HCC− (P<0.01). The Glutathione species and its ratio from the RBC compartment were similar among all groups. OA concentration in both plasma and RBC was significantly higher from WHV+/HCC+ when compared to WHV+/HCC− and WHV−/HCC− (P<0.01). Conclusions. Disturbances of the glutathione redox buffer system and higher concentrations of OA were found in the WCV+/HCC+ animal model. The role of these compounds as biomarkers of early tumor development in patients with end stage liver disease remains to be determined.