HPB Surgery

HPB Surgery / 2012 / Article / Fig 8

Research Article

Ischemic Preconditioning of Rat Livers from Non-Heart-Beating Donors Decreases Parenchymal Cell Killing and Increases Graft Survival after Transplantation

Figure 8

Decreased parenchymal and nonparenchymal cell killing by ischemic preconditioning after aortic clamping and cold storage with and without reperfusion. Anesthesized rats were given a sham operation or ischemic preconditioning (IP) by clamping of the hepatic artery and portal vein for 10 min followed by 5 min of blood reperfusion. The aortas of the rats were then clamped for 60 min. After clamping, the livers were stored for 2 h in cold UW solution. Subsequently, the livers were infused for 5 min with cold UW solution containing trypan blue to label nonviable cells in the absence of warm reperfusion (unreperfused) or infused for 15 min with warm KHB containing trypan blue to label nonviable cells after warm reperfusion (reperfused), as described in section 2. Nonviable parenchymal cells (a) and nonparenchymal cells (b) were counted from 5 livers per group. * compared to the corresponding non-IP group by ANOVA.

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