The Prognostic Significance of Lymphatics in Colorectal Liver Metastases

Figure 3

LYVE-1 not able to detect lymphangiogenesis in tumor. (a) Paraffin embedded section showing lymphatic vessels stained with D2-40 (×80). (b) Magnified insert highlighting the strong staining of D2-40 expressing lymphatics within the tumor (arrows) (×400). (c) D2-40 did not stain the liver sinusoids or hepatic blood vessels (arrow) (×400). (d) Serial sections stained using immunohistochemistry with LYVE-1; revealed LYVE-1 was not a specific marker for lymphangiogenesis in the liver (×80). (e) LYVE-1 was not able to detect lymphatic vessels in the tumor periphery where D2-40 was able to detect lymphatic vessels (arrows) (×400). (f) LYVE-1 was expressed in liver sinusoids and hepatic blood vessels (arrows) (×400).