Infectious Diseases in Obstetrics and Gynecology

Infectious Diseases in Obstetrics and Gynecology / 1994 / Article

Clinical Study | Open Access

Volume 2 |Article ID 436937 |

Anil K. Sood, Zahra Bahrani-Mostafavi, Jay Stoerker, I. Keith Stone, "Human Papillomavirus DNA in LEEP Plume", Infectious Diseases in Obstetrics and Gynecology, vol. 2, Article ID 436937, 4 pages, 1994.

Human Papillomavirus DNA in LEEP Plume

Received22 Jun 1994
Accepted09 Sep 1994


Objective: This study was undertaken to determine the prevalence of human papillomavirus (HPV) in loop electrosurgical excision procedure (LEEP) plumes.Methods: Forty-nine consecutive patients with colposcopic and cytologic evidence of cervical intraepithelial neoplasia (CIN) were tested. Smoke plumes were collected through a filter placed in the suction tubing. DNA was harvested by proteinase K digest of the filters and prepared for polymerase chain reaction (PCR) by L1 consensus primers.Results: Thirty-nine (80%) tissue samples were positive for HPV, with types 6/11 in 4, 16/18 in 19, 31/33/35 in 2, and other types in 6 patients. The tissue sample was inadequate for typing in 8 patients. HPV DNA was detected in 18 (37%) filters.Conclusions: Although the consequences of HPV in LEEP plume are unknown, it would be prudent to adopt stringent control procedures.

Copyright © 1994 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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