Objective: This study was planned to clarify the in vitro effect of
lidocaine and bupivacaine on germ tube formation by Candida albicans isolates from
cases of clinical vaginal candidiasis.Methods: Fourteen C. albicans strains (clinical vaginal isolates)
were grown on Sabouraud agar for 24 h at 37℃ and tested as follows: 100 μl of a yeast
suspension [105
colony forming units (CFU)/ml of phosphate buffered saline (PBS)] was
added to 500 μl of fresh human serum with lidocaine or bupivacaine (pure salts) in serial
concentrations. The test was run in duplicate. Controls were prepared for each strain.
After 4 h of incubation at 37℃, samples were taken from each vial and 200 yeasts were
counted in a counting chamber. The pH of each suspension was measured.Results: The results are given as the mean of the 2 readings and are
expressed as the percentage of blastoconidia with germ tubes/total blastoconidia.Conclusions: Our experiments show that both lidocaine and
bupivacaine have a dose-dependent inhibitory effect, pH-independent, on germ tube
formation by C. albicans and that both drugs seem to be promising in the treatment of
genital candidiasis due to the combination of anesthetic and antifungal properties.