Table of Contents Author Guidelines Submit a Manuscript
Infectious Diseases in Obstetrics and Gynecology
Volume 5 (1997), Issue 3, Pages 226-231
http://dx.doi.org/10.1155/S1064744997000380
Clinical Study

Evaluation of a Fast Test to Identify the Presence of Proline Aminopeptidase in Women With Bacterial Vaginosis

1Obstetrics and Gynecology Clinic, Hospital Juárez de México, Mexico City, Mexico
2Research Center on Infectious Diseases, National Public Health, Cuernavaca, Morelos, Mexico
3Rodríguez Saro N. 523, Department 104-A, Col. Del Valle, C.P. 03100, México D.F., Mexico

Received 27 December 1996; Accepted 21 April 1997

Copyright © 1997 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Objective: The purpose of this study was to evaluate the activity of proline aminopeptidase by a rapid paper strip test in women with bacterial vaginosis (BV).

Methods: Vaginal secretions of 1,387 voluntary patients attending the Obstetrics and Gynecology Infectious Diseases Clinic at Juárez Hospital of Mexico City were collected and examined. Patients were assigned into 2 groups: 483 with BV according to clinical and laboratory criteria and 604 without BV as the control group. For the purposes of this study, 300 patients with trichomonas and/or yeast were excluded from the BV group. The strips were prepared by using L-proline β-naphthylamide and L-proline p-nitroanilide as the substrates to detect proline aminopeptidase activity in concentrated vaginal secretions. In parallel, all samples were also analyzed with the standard methods in microplates containing either sustrate as a control of the rapid strip test. The test was interpreted after 3–5 min of incubation.

Results: The results in the strip and microplate assays were similar in 95% of the samples. Sensitivity was 91.7% and specificity was 94.2%; probability of BV if the test is positive was 92.6% and negative predictive value was 93.4%.

Conclusions: These findings indicate that this aminopeptidase rapid strip assay provides a 3–5 min identification of activity of the enzyme in women with BV. The procedure is a rapid, non-expensive, sensitive, and useful test at the gynecologic clinic.