Infectious Diseases in Obstetrics and Gynecology

Infectious Diseases in Obstetrics and Gynecology / 2001 / Article

Open Access

Volume 9 |Article ID 284191 | https://doi.org/10.1155/S1064744901000199

David M. Svinarich, Fadi M. Zaher, Lena Holmdahl, Nasser Chegini, Bernard Gonik, Michael P. Diamond, "Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase", Infectious Diseases in Obstetrics and Gynecology, vol. 9, Article ID 284191, 4 pages, 2001. https://doi.org/10.1155/S1064744901000199

Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase

Received28 Nov 2000
Accepted07 Mar 2001

Abstract

Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels.Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × 10-4, ≤ 3.12 × 10-4, 6.24 × 10-4 and 2.5 × 10-3 attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × 10-1 and 6.25 × 10-2 attomoles/μl.Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.

Copyright © 2001 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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