David M. Svinarich, Fadi M. Zaher, Lena Holmdahl, Nasser Chegini, Bernard Gonik, Michael P. Diamond, "Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase", Infectious Diseases in Obstetrics and Gynecology, vol. 9, Article ID 284191, 4 pages, 2001. https://doi.org/10.1155/S1064744901000199
Adhesion Development and the Expression of Endothelial Nitric Oxide Synthase
Objective: This study was conducted to determine whether nitric oxide (NO), a potent vasodilator and inhibitor of thrombus formation, is involved in the formation and maintenance of adhesions.Methods: Skin, subcutaneous tissues, peritoneum and adhesions were collected from surgical patients and total RNA was isolated. Quantitative reverse transcription polymerase chain reaction (QRT-PCR) was performed to quantitate endothelial nitric oxide synthase (eNOS) and β-actin mRNA levels.Results: eNOS mRNA levels for skin, subcutaneous tissue, peritoneum and adhesions were ≤ 3.12 × , ≤ 3.12 × , 6.24 × and 2.5 × attomoles/μl, respectively. β-actin mRNA levels for all tissues were between 1.25 × and 6.25 × attomoles/μl.Conclusion: eNOS mRNA can be identified in tissue adhesions, and may therefore play a role in adhesion formation and maintenance.
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