Roger E. Bawdon, Victor Ghetie, "The Bidirectional Transfer and Fetal Vascular Pressure Changes Due to the Presence of I-Labeled Inhibin A in the ex-vivo Human Placental Model", Infectious Diseases in Obstetrics and Gynecology, vol. 11, Article ID 704536, 4 pages, 2003. https://doi.org/10.1080/10647440300025505
The Bidirectional Transfer and Fetal Vascular Pressure Changes Due to the Presence of I-Labeled Inhibin A in the ex-vivo Human Placental Model
Objective: The purpose of this study was to investigate the transport of inhibin A and to determine its effects on fetal vascular pressure at elevated levels in the human placenta using -labeled synthetic glycoprotein.Methods: Synthetic inhibinAwas prepared and was shown to be consistent with the natural form by high-pressure liquid chromatography (HPLC) and molecular weight determination by gas-chromatography mass spectrometry. The standardized Na process yielded -labeled inhibin A with a radioactivity of cpm/μg. This compound was placed in the human placenta in maternal–fetal and fetal–maternal studies using antipyrine and -labeled inulin as controls to determine the bidirectional transfer of the compound.Results: Maternal–fetal and fetal–maternal clearance indices were 0.045± 0.003 and 0, respectively. In eight placentas there was no evidence of vascular pressure changes due to the presence of up to 5000 pg of inhibin A.Conclusions: There is minimal maternal–fetal transfer and no detectable fetal–maternal transfer in normotensive and pregnancy-induced hypertensive placentas. In addition, there are no pressure changes in the fetal vascular system due to the clinically significant levels of inhibin A.
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