Infectious Diseases in Obstetrics and Gynecology

Infectious Diseases in Obstetrics and Gynecology / 2005 / Article

Open Access

Volume 13 |Article ID 961356 | https://doi.org/10.1155/2005/961356

Michael Hnat, Roger E. Bawdon, "Transfer of Meropenem in the ex Vivo Human Placenta perfusion Model", Infectious Diseases in Obstetrics and Gynecology, vol. 13, Article ID 961356, 5 pages, 2005. https://doi.org/10.1155/2005/961356

Transfer of Meropenem in the ex Vivo Human Placenta perfusion Model

Abstract

Objectives. To determine maternal-fetal transplacental passage of meropenem in the ex vivo human perfusion model.Study design. Term placentae (n = 6) were collected immediately after delivery. A single cotyledon was localized, perfused and stabilized with physiologic Eagles minimal essential medium containing 3% bovine albumin and heparin as described by Chalier (Chalier JC. Criteria for evaluating perfusion experiments and presentation results. Contrib Gynecol Obstet 1985; 13:32–39). Meropenem was added to the maternal medium in concentrations similar to maternal serum peak and trough levels, then perfused through the maternal circulation of the cotyledon. To assess transfer and accumulation, fluid aliquots from both the maternal and fetal compartments were collected over an hour at defined intervals in an open and closed system. Antipyrine C14 was added to the medium in order to calculate the transport fraction and clearance indexes. Meropenem and antipyrine C14 concentrations were determined by High-pressure Liquid Chromatography and liquid scintillation, respectively.Results. Mean antipyrine transport fraction was 2.33 + 0.25. Maternal and fetal mean meropenem peak concentrations were 54.3 + 3.3 μg/ml and 2.2 + 0.18 μg/ml, respectively. Whereas, maternal and fetal mean trough concentrations were 12.7 + 1.3 μg/ml and 0.41 + 0.10 μg/ml, respectively. Mean peak clearance index was 0.077 + 0.007 and the mean trough was 0.052 + 0.015. Mean accumulation for the peak and trough concentrations of meropenem were 0.9 and 2.95 μg/ml, respectively.Conclusions. Transplacental passage of meropenem was incomplete in the ex vivo human placental perfusion model. Accumulation was also noted in the fetal compartment

Copyright © 2005 Hindawi Publishing Corporation. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


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