Abstract
Background. Pathogenesis of mucosal microorganisms
depends on adherence to the tissues they colonize and infect. For
Candida albicans, cell surface hydrophobicity may play a
significant role in tissue binding ability. Methods. A
continuous cell line of vaginal epithelial cells (VEC)
was grown in keratinocyte serum-free medium (KSFM) with
supplements and harvested by trypsinization. VEC were combined
with yeast cells to evaluate adherence and inhibition of
adherence. In this experimental setup, yeast stained with
fluorescein isothiocyanate were allowed to attach to VEC and the
resulting fluorescent VEC were detected by flow cytometry.
Results. VEC were cultured and examined daily after
plating and showed morphology similar to basal epithelial cells.
Culture media supplemented with estradiol showed increased VEC
proliferation initially (first