Infectious Diseases in Obstetrics and Gynecology / 2009 / Article / Fig 3

Research Article

Pretreatment with Pancaspase Inhibitor (Z-VAD-FMK) Delays but Does Not Prevent Intraperitoneal Heat-Killed Group B Streptococcus-Induced Preterm Delivery in a Pregnant Mouse Model

Figure 3

Intrauterine HK-GBS injection leads to caspase 3 activation in the membranes and placenta. Day 14.5 timed pregnant mice were injected with either PBS or HK-GBS and euthanized at 5 or 14 hours to isolate fetal membranes (a–c) and placentas (d–f). Caspase 3 activation was assessed by performing immunohistochemistry analysis using an antibody against active-cleaved caspase 3. Representative data from three separate experiments are shown. HK-GBS exposure led to an increase in caspase 3 positive cells in the membranes (c) and above the spongiform trophoblast layer at 14 hours in the placenta (f).
749432.fig.003a
(a) PBS
749432.fig.003b
(b) HK-GBS (5 hours)
749432.fig.003c
(c) HK-GBS (14 hours)
749432.fig.003d
(d) PBS
749432.fig.003e
(e) HK-GBS (5 hours)
749432.fig.003f
(f) HK-GBS (14 hours)

Article of the Year Award: Outstanding research contributions of 2020, as selected by our Chief Editors. Read the winning articles.